Chem 471/671				Quiz 9				
Chapter 9   DNA Tech
Due on Blackboard by Monday Nov 13 at 5pm.

1.	The biological role of restriction enzymes is to: 
A) aid recombinant DNA research. 
B) degrade foreign DNA that enters a bacterium. 
C) make bacteria resistant to antibiotics. 
D) restrict the damage to DNA by ultraviolet light. 
E) restrict the size of DNA in certain bacteria. 

2. The enzyme Bam H1 cleaves the sequence GGATCC. Approximately how many fragments would you expect to get after a complete digestion of a 20 kilobase pair double stranded DNA fragment from i) a eukaryotic genome and ii) the Bacillus amyloliquifaciens H genome. 
A)  i 1 	 and 	ii 1
B)  i <10	 and 	ii 1
C)  i >100 and 	ii >100 
D)  i <10	 and 	ii >100
E)  i 1	 and 	ii >100
  
3. Which of the following enzymes use(s) ATP as a substrate
A)  Alkaline phosphatase
B)  DNA ligase
C)  DNA polymerase
D)  Reverse transcriptase
E)  Both DNA polymerase and Reverse transcriptase

4. The PCR reaction mixture does not include: 
A) all four deoxynucleoside triphosphates. 
B) DNA containing the sequence to be amplified. 
C) DNA ligase. 
D) heat-stable DNA polymerase. 
E) oligonucleotide primer(s). 

5. Genomic and cDNA libraries have the following characteristics:
A)  cDNA clones can be assembled into contigs
B)  ESTs are present in both cDNA and genomic libraries
C)  ESTs are present in cDNA libraries only
D)  ESTs are present in genomic libraries only
E)  genomic clones are better for protein expression libraries
 
6. Southern Blotting 
A)  Requires labeling of a DNA probe applied after electrophoresis
B)  Requires labeling of an RNA probe applied after electrophoresis
C)  Requires labeling of DNA prior to electrophoresis
D)  Requires labeling of either a DNA or an RNA probe applied after electrophoresis
E)  Requires labeling of RNA prior to electrophoresis


7. A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features except:
A) a selectable antibiotic marker such as kanamycin resistance.
B) a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice.
C) a Ti plasmid lacking its T-DNA segment.
D) active vir gene products from the altered Ti plasmid.
E) an ability to induce crown gall formation in infected leaves.

8. DNA microarrays allow for assays of cellular:
A)  Both protein and RNA levels
B)  Both RNA and DNA levels
C)  DNA levels
D)  Protein levels
E)  RNA levels

9. The 25bp repeats that flack the T-DNA of agrobacterium are similar functionally to the:
A)  Antibiotic resistance gene of pBR322
B)  Bacterial artificial chromosome ori sequence
C)  Bacteriophage lambdas packaging signals 
D)  Retroviral Long Terminal Repeats 
E)  Yeast artificial chromosome TEL sequences
 
10. Novel genes that produce proteins never before found in nature, can be produced by
A)  Site directed mutagenesis of an expression vector
B)  Screening a cDNA library
C)  PCR
D)  Transgenic Organisms
E)  Two of the above answers are correct.