Instructor: Michael C. Mossing, 452 Coulter, 915-5339, mmossing@olemiss.edu
Laboratory Schedule: 1 to 4 pm, MTuWTh 404 Coulter, (Most experiments require 1 or 2 days)
Lecture Schedule: Fri 2 - 2:50 pm, 200 Coulter (not every Friday, occasional)
Text: Fundamental Laboratory Approaches to Biochemistry and Biotechnology
A.J. Ninfa and D. Ballou, (1998), Fitzgerald Science Press, Inc., Bethesda, MD
URL: http://www.olemiss.edu/depts/chemistry/courses/chem472/
Syllabus in (PDF | MSWord) Teaching Assistants: Lei Wang and Yin Gu

This laboratory course in biochemistry will provide hands-on instruction in basic biochemical techniques. We will focus on the production, purification, and analysis of proteins and nucleic acids. After a series of textbook experiments to get familiar with standard biochemical techniques, Each lab group will research, design and test one laboratory experiment. A detailed experimental protocol and model lab report will count for 20% of the grade for the course. The remaining 80% will be determined by 10 laboratory reports.

In addition, those enrolled in the graduate course Chemistry 672 will be required to perform an integrated long-term experiment of their choice throughout the course of the semester. Experiments might include, modification and cloning of a gene for protein production in E. coli, followed by expression and purification of the desired protein.

Required materials:

1. Textbook: Fundamental Laboratory Approaches for Biochemistry and Biotechnology by Alexander J. Ninfa and David P. Ballou
2. Bound laboratory notebook (from Scientific Notebook - available in the stockroom).
3. Safety Goggles.
4. PC-formatted floppy disk for data files

Sample protocols and lab report outlines from 2005 are available at: http://www.olemiss.edu/depts/chemistry/courses/chem472_05/