Experiment 6a - PCR sequences

Last Updated: Tuesday, 20-Mar-2007 12:01:46 CDT

PCR primers

Paste the following lines into the primers box on the Sequence Extractor page

ccaGGCATCACGGCAATATAC P1,
ggcTCTGGTCTGGTAGCAATG P4,
aacACTTAACGGCTGACATGG P2,
aACGAGTATCGAGATGGCAca P3,
TTttTtatActAA AttCoreF,
TTagTataAaaAA AttCoreR

DNA sequences

Open the links below and copy the DNA sequences into the template box on the Sequence Extractor page

• pAH125 contains the lambda attachment site its complete sequence (5739 bp) is available at Genbank
• This small fragment of 845 bp contains the lambda Att site pAH125_3288-4132.fasta
• This small fragment of 925 bp contains the bacterial Att site AttB.fasta
• Here is the sequence of flanking bacterial sequences and integrated pAH125 (5739 + 925 bp). integratedpAH125.fasta