Instructor: Michael C. Mossing, 452 Coulter, 915-5339,mmossing@olemiss.edu
Laboratory Schedule: 1 to 4 pm, MTuWTh 404 Coulter, (Most experiments require 1 or 2 days)
Lecture Schedule: Fri 2 - 2:50 pm, 200 Coulter (not every Friday, occasional)
Text: Fundamental Laboratory Approaches to Biochemistry and Biotechnology
A.J. Ninfa and D. Ballou, (1998), Fitzgerald Science Press, Inc., Bethesda, MD
URL: http://www.olemiss.edu/depts/chemistry/courses/chem472/
Syllabus in (PDF | MSWord)
Teaching Assistants: Lei Wang and Yin Gu
Last Updated: Thursday, 07-Apr-2005 15:50:57 CDT
At 12:34 PM on 2005.03.31. Protocols posted. If your protocol is not here please email it to me ASAP. There are also hard copies in a notebook above the Gel Doc. You should have a hard copy along with the notebook at your lab station. Each group will be responsible for doing 3 protein experiments (choose from P1-P4) and 3 DNA experiments (choose from D1-D4) by the end of the semester.
At 2:57 PM on 2005.01.27. Lab Report Outlines (RTF | PDF) for experiment 1 posted.
| Expt | Topic | Developers |
| D1 | DNA purification, DNA Restriction Analysis and Electrophoresis
DOC | PDF Links to DNA sequence files | Loftin, Majori, Powell |
| D2 | Ligation and Transformation DOC | PDF | Bell, Hutto |
| D3 | PCR
DOC | PDF Links to DNA sequence files | Greene, Johnson, Tran, Vunnam |
| D4a | Forensic STR analysis - PCR DOC | PDF | Allen, Herron, Richmond, Smith |
| D4b | Forensic STR analysis - PAGE DOC (32 Mb) | PDF | Chung, Leslie, Marshall |
| P1 | Ion Exchange Chromatography of Lysozyme DOC | PDF | Mosley, Nelson, Seely |
| P2 | Purification of βgalactosidase DOC | PDF | Anazia, Butler, Song |
| P3 | SDS Polyacrylamide Gel Electrophoresis DOC ( Warning! 32 Mb) | PDF (1.9 Mb) | Lucas, McMorries, Graham |
| P4 | Equilibrium DOC | PDF | Bernard, Grady |
This laboratory course in biochemistry will provide hands-on instruction in basic biochemical techniques. We will focus on the production, purification, and analysis of proteins and nucleic acids. After an initial textbook experiment to refresh our memories concerning chemical solutions (dilutions, calculations, pH) and get familiar with standard biochemical techniques, Each lab group will research, design and test one laboratory experiment. A detailed experimental protocol and model lab report will count for 50% of the grade for the course (25% determined by the instructor, 25% determined by your peers who will rotate through your experiment for the rest of the semester). The remaining 50% will be determined by 10 laboratory reports on experiments that others have designed.
In addition, those enrolled in the graduate course Chemistry 578 will be required to perform an integrated long-term experiment of their choice throughout the course of the semester. Experiments might include, modification and cloning of a gene for protein production in E. coli, followed by expression and purification of the desired protein.
Required materials:
Sample protocols and lab report outlines from last year are available are available at: http://www.olemiss.edu/depts/chemistry/courses/chem472_04/